Journal: Molecular medicine reports

Article Title: Saururus chinensis (Lour.) Baill. extract promotes skeletal muscle cell differentiation by positively regulating mitochondrial biogenesis and AKT/mTOR signaling in vitro .

doi: 10.3892/mmr.2024.13250

Figure Lengend Snippet: Figure 6. SCE regulates AKT/mTOR and its downstream effectors in myotubes. (A) C2C12 myoblasts were induced to differentiate in a DMEM containing 2% horse serum, treated for 5 days with different concentration of SCE (1, 5 and 10 ng/ml) and analyzed using western blotting (left). Quantification of protein expression levels (n=3 per group) (right). **P<0.01 and ***P<0.001 vs. the control. (B) Cells were treated with 10 ng/ml SCE in DM for 1, 3 and 5 days and western blotting was performed using the indicated antibodies (left). Quantification of protein expression levels (n=3 per group) (right). *P<0.05, **P<0.01 and ***P<0.001 vs. the DMSO; #P<0.05, ##P<0.01 and ###P<0.001 vs. the DMSO at the indicated time points. SCE, Saururus chinensis (Lour.) Baill. extract; p‑, phosphorylated; p70S6K1, ribosomal protein S6 kinase B1.

Article Snippet: Specific antibodies against myosin heavy chain (MyHC; 1:500; cat. no. sc‐376157), myogenic differen‐ tiation 1 (MyoD; 1:1,000; cat. no. sc‐377460), myogenin and peroxisome proliferator‐activated receptor‐gamma coactivator‐1 α (Pgc‐1α; 1:1,000; cat. no. sc‐518038) were obtained from Santa cruz Biotechnology, inc. antibodies against non‐phospho (active) β‐catenin (1:1,000; cat. no. 8814), β‐catenin (1:1,000; cat. no. 9582), phospho‐aMPK (1:1,000; cat. no. 2535), aMPK (1:1,000; cat. no. 2532), phospho‐AKT (1:1,000; cat. no. 9271), AKT (1:1,000; cat. no. 9272), phospho‐mTOR (1:1,000; cat. no. 2971), mTOR (1:1,000; cat. no. 2983), phospho‐ribosomal protein S6 kinase B1(p70S6K1) (1:1,000; cat. no. 9234), p70S6K1 (1:1,000; cat. no. 2708) and glyceraldehyde 3‐phos‐ phate dehydrogenase (gaPdH; 1:1,000; cat. no. 2118) were purchased from Cell Signaling Technology, Inc.

Techniques: Concentration Assay, Western Blot, Expressing, Control

Journal: BMC Cancer

Article Title: HRG-β1-driven ErbB3 signaling induces epithelial–mesenchymal transition in breast cancer cells

doi: 10.1186/1471-2407-13-383

Figure Lengend Snippet: Knockdown of ErbB3 suppresses HRG-β1-induced EMT in SK-BR-3 cells. The cells were transfected with control (Ctrl) or ErbB3 siRNAs and treated with 25 ng/ml of HRG-β1 for 24 h. The expressions of ErbB3, E-cadherin, fibronectin, phospho-Smad2, and Snail were analyzed by western blotting. β - actin was reprobed as a loading control. Data represent the means ± SD of three independent experiments. * P < 0.05, ** P < 0.01, significant difference.

Article Snippet: For transfection, the cells were grown to confluence in 6-cm plates and a Smad2 siRNA (Santa Cruz Biotechnology Inc.) and a ErbB3 siRNA at 60 pmol (Santa Cruz Biotechnology Inc.) were transfected using a siRNA transfection reagent (Santa Cruz Biotechnology Inc.) according to the manufacturer’s instructions.

Techniques: Knockdown, Transfection, Control, Western Blot